Shikonin
作者：齐岳
发布时间：2023-09-06
09:05:33
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产品名称：Shikonin
产品描述：
| 植物来源 | 天然产物 > 其他（植物来源） > 其他 |
| 产品描述 | Shikonin is an TMEM16A chloride channel inhibitor (IC50: 6.5 μM). Shikonin is also a selective PKM2 inhibitor and can also suppress activation of the NF-κB pathway and inhibit TNF-α. Shikonin decreases exosome secretion through the inhibition of glycolysis |
| 靶点活性 | PKM2 (FBP presence):0.8 μM, PKM2 (FBP absence):0.3 μM, TMEM16A chloride channel:6.5 μM |
| 体外活性 | Shikonin (>50 μM) markedly inhibits normal human keratinocytes (NHKs) viability, compare with that of control (P<0.05). Pretreatment with Shikonin for 2 h attenuates TNF-α-induced NF-κB p65 nuclear translocation[3]. Shikonin (5/7.5 μM) markedly inhibits the cell viability starting from 12 h and the inhibitory effects are presented in time-dependent patterns compare with the 0 h group in both cell lines. It is found that 5 μM Shikonin displays greater inhibition compare to 2.5 μM at the time points from 24 to 48 h. The invasiveness of U87 and U251 cells is significantly attenuated when treated with Shikonin (2.5/5/7.5 μM) compare with the control group at 24 and 48 h (p<0.01)[4]. |
| 体内活性 | Shikonin markedly inhibits the increase in TNF-α and IL-1β expression levels in the rat model of osteoarthritis, compared with those in the osteoarthritis group (P<0.01). The NF-κB protein expression level is significantly suppressed by Shikonin in the rat model of osteoarthritis, compare with that in the osteoarthritis group (P<0.01). The induction of the iNOS level is suppressed by treatment with Shikonin in the rat model of osteoarthritis, compare with that in the osteoarthritis group (P<0.01). The administration of Shikonin markedly weakens the up-regulation of COX-2 protein expression in the rat model of osteoarthritis, as compared with that in the osteoarthritis group (P<0.01). The elevation of caspase-3 activity is significantly reduced by Shikonin treatment in the rat model of osteoarthritis, compare with that in the osteoarthritis group (P<0.01). The downregulation of Akt phosphorylation is also significantly recovered by treatment with Shikonin in the rat model of osteoarthritis, compared with that in the osteoarthritis group (P<0.01)[5]. |
| 细胞实验 | U87 and U251 cells are seeded into 96-well plates at a density of 1×104 cells per well in standard DMEM and incubated for 24 h under standard conditions (37°C and 5% CO2). Then the medium is replaced with either blank, serum-free DMEM or DMEM containing Shikonin at concentrations of 2.5, 5, and 7.5 μM. The total volume in each well is 200 μL. Finally, the plates are shaken softly and the optical density is recorded at 570 nm (OD570) using a plate reader. At least three independent experiments are performed[4]. |
| 动物实验 | Healthy male Sprague-Dawley rats (n=30; 8 to 10-weeks old, 250 to 300 g) are used in this study. Rats were randomly assigned to three groups: Sham-operated group (n=10), osteoarthritis model group (n=10) and Shikonin-treated group (n=10). In the sham-operated group, the right knee joint of the anesthetized rat is only exposed under sterile conditions, and the rats are treated with 0.1 ml/100 g physiological saline (i.p.). In the osteoarthritis model group, osteoarthritis model rats were treated with 0.1 ml/100 g physiological saline (i.p.). In the Shikonin-treated group, osteoarthritis model rats are treated with 10 mg/kg Shikonin (i.p.) once daily for 4 days after osteoarthritis modeling[5]. |
| 别名 | C.I. 75535, (+)-Shikonin, Alkanna Red, 紫草素, NSC 252844, Anchusa acid, Isoarnebin 4 |
| 分子量 | 288.3 |
| 分子式 | C16H16O5 |
| CAS No. | 517-89-5 |
存储
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: 57 mg/mL
H2O: Insoluble
Ethanol: 13 mg/mL(45.1 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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