Ac-DEVD-AMC
作者：齐岳
发布时间：2023-08-29
13:40:50
点击数：
产品名称：Ac-DEVD-AMC
产品描述：
| 产品描述 | Ac-DEVD-AMC is the substrate of Caspase-3 . |
| 体外活性 | The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2]. |
| 细胞实验 | Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2]. |
| 别名 | AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN, AC-ASP-MET-GLN-ASP-AMC |
| 分子量 | 675.64 |
| 分子式 | C30H37N5O13 |
| CAS No. | 169332-61-0 |
存储
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: ≤10mg/ml(14.80 mM)
H2O: < 0.1 mg/mL (insoluble)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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