JIB-04
作者：齐岳
发布时间：2023-09-06
08:59:45
点击数：
产品名称：JIB-04
产品描述：
| 产品描述 | JIB-04 is a pan-selective Jumonji histone demethylase inhibitor. |
| 靶点活性 | JARID1A:230 nM, JMJD2B:435 nM, JMJD2E:340 nM, JMJD2D:290 nM, JMJD2A:445 nM |
| 体外活性 | 在H358和A549小鼠异种移植模型中,JIB-04抑制Jumonji组蛋白脱甲基酶的活性,抑制肿瘤细胞生长. |
| 体内活性 | 在肺癌和前列腺癌细胞系中（IC50=10 nM）,JIB-04下调增殖基因,阻断细胞生长。 |
| 细胞实验 | For cell viability assays, cells are plated at 1500-3000 cells/well in 96 well plates and treated the next day with increasing doses of compound over 4 days and their viability assessed by standard MTS assays using Promega’s Cell Titer or Cell Titer-Glo reagents according to the manufacturer’s protocols. Absorbance at 490 nm and 650 nm or luminescence is measured by a Spectra Max or a FlouroStar Omega plate reader. Data are normalized to the untreated controls (100% viability). Each cell line is tested in 2-5 independent assays, each containing 4-8 replicates. IC50 values are calculated using DIVISA, a high-throughput software, developed in hous, for storing and analyzing drug sensitivity assays. Dose-response curves are plotted using a non-linear regression model and IC50s are determined from the fitted curves. The average IC50 derived from 2-5 independent assays, each containing 4-8 replicates is reported. (Only for Reference) |
| 别名 | NSC 693627, 5-氯-2(1H)-吡啶酮 (2E)-(苯基-2-吡啶基亚甲基)腙, JIB 04 |
| 分子量 | 308.76 |
| 分子式 | C17H13ClN4 |
| CAS No. | 199596-05-9 |
存储
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: 30.9 mg/mL (100 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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公司介绍：
西安齐岳生物科技有限公司是集化学科研和定制与一体的高科技化学公司。业务范围包括化学试剂和产品的研发、生产、销售等。涉及产品为通用试剂的分销、非通用试剂的定制与研发，涵盖生物科技、化学品、中间体和化工材料等领域。
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